Human IL-31 DTSet enzyme-linked immunoassay kit
Catalog# DSEH009705；DSEH009715
Human IL-31 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Overview
Product Name
Human IL-31 DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0147 | 0.0145 | 0.0146 | |
| 15.63 | 0.0501 | 0.0491 | 0.0496 | 0.0350 |
| 31.25 | 0.1059 | 0.1101 | 0.1080 | 0.0934 |
| 62.50 | 0.2132 | 0.1990 | 0.2061 | 0.1915 |
| 125.00 | 0.4529 | 0.4563 | 0.4546 | 0.4400 |
| 250.00 | 1.0150 | 1.0140 | 1.0145 | 0.9999 |
| 500.00 | 2.1110 | 2.2760 | 2.1935 | 2.1789 |
| 1000.00 | 3.7770 | 3.7080 | 3.7425 | 3.7279 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Human
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
15.63 pg/ml-1000 pg/ml
Standard Curve Gradient
7点/3倍
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
Target
UNIPROT
Synonyms
IL 31
IL-31
IL31
IL31_HUMAN
Interleukin 31
Interleukin-31
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