Human ULBP1/NKG2DL1 DTSet enzyme-linked immunoassay kit
Catalog# DSEH015005；DSEH015015
Human ULBP1/NKG2DL1 DTSet enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Overview
Product Name
Human ULBP1/NKG2DL1 DTSet enzyme-linked immunoassay kit
Product Type
DTSet Elisa Kit
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0245 | 0.0226 | 0.0236 | |
| 7.81 | 0.1904 | 0.1863 | 0.1884 | 0.1648 |
| 15.63 | 0.3245 | 0.3320 | 0.3283 | 0.3047 |
| 31.25 | 0.5924 | 0.5929 | 0.5927 | 0.5691 |
| 62.50 | 1.1300 | 1.1560 | 1.1430 | 1.1195 |
| 125.00 | 2.0150 | 1.9480 | 1.9815 | 1.9580 |
| 250.00 | 3.1300 | 3.0630 | 3.0965 | 3.0730 |
| 500.00 | 4.0490 | 4.1558 | 4.1024 | 4.0789 |
Experiment Type
Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)
Species Reactivity
Human
Detection Method
Colorimetric Method
Experiment Detection Duration
120min
Standard Curve Range
7.81 pg/ml -500 pg/ml
Standard Curve Gradient
7点/3倍
Number of Incubations
2
Detectable Sample
Sample Loading Volume
50 μl
Product Features
- Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
- A development protocol is provided to guide further analysis optimization.
- Detection can be customized according to your specific needs.
- An economical alternative to a complete kit.
Kit Components
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin-HRP Conjugate
Other Required Materials
DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.
The components listed above can be purchased separately.
- 96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
- Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
- Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
- Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
- Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
- Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
- Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
- Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。
Target
UNIPROT
Synonyms
UL16-binding protein 1、ALCAN-beta、NKG2D ligand 1、N2DL-1、NKG2DL1
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