Human Leptin DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Bulk Order Quote | Customization Request

Catalog# DSEH005605；DSEH005615

Human Leptin DTSet enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Overview

Product Name

Human Leptin DTSet enzyme-linked immunoassay kit

Product Type

DTSet Elisa Kit

pg/ml	O.D.		Average	Corrected
0.00	0.0405	0.0421	0.0413
31.25	0.1459	0.1363	0.1411	0.0998
62.50	0.2217	0.2166	0.2192	0.1779
125.00	0.3683	0.3594	0.3639	0.3226
250.00	0.7537	0.6959	0.7248	0.6835
500.00	1.1770	1.1870	1.1820	1.1407
1000.00	2.0620	2.0120	2.0370	1.9957
2000.00	3.3900	3.2800	3.3350	3.2937

Experiment Type

Sandwich Enzyme-Linked Immunosorbent Assay (Sandwich Elisa)

Species Reactivity

Human

Detection Method

Colorimetric Method

Experiment Detection Duration

120min

Standard Curve Range

31.25 pg/ml-2000 pg/ml

Standard Curve Gradient

7点/3倍

Number of Incubations

Detectable Sample

Sample Loading Volume

50 μl

Product Features

Optimized pairing of capture and detection antibodies with recommended concentrations can save a long development time.
A development protocol is provided to guide further analysis optimization.
Detection can be customized according to your specific needs.
An economical alternative to a complete kit.

Kit Components

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin-HRP Conjugate

Other Required Materials

DTSet Assistant Kit (5 plates): Includes 96-well microplates, plate sealing films, color development solution, termination solution, plate coating buffer (PBS), washing buffer, and detection buffer.

The components listed above can be purchased separately.

96-well Microplate (EP): YOUKE Life, Catalog # DSEP01
Plate Sealing Film (PS): YOUKE Life, Catalog # DSSF01。
Plate Coating Buffer (CB): 137mM NaCl，2.7mM KCl，8.1mM Na2HPO4，1.5mM KH2PO4，pH 7.2-7.4，0.2m过滤。YOUKE Life, Catalog # DSCB01。
Detection Buffer 10X (AB): 0.5%BSA，0.05%Tween20，PBS溶液。YOUKE Life, Catalog # DSAB01。
Blocking Solution (BB): YOUKE Life, Catalog # DSBB01。
Washing Solution: 0.05%Tween20，PBS溶液，pH 7.2-7.4。YOUKE Life, Catalog # DSWB01。
Substrate Color Development Solution (TMB): TMB。YOUKE Life, Catalog # DSTS01。
Termination Solution (SS): 0.5mol/ml H2SO4。YOUKE Life, Catalog # DSSS01。

Target

UNIPROT

P41159

Synonyms

FLJ94114

LEP

LEP_HUMAN

LEPD

Leptin (murine obesity homolog)

Leptin (obesity homolog

mouse)

Leptin

Leptin Murine Obesity Homolog

Leptin Precursor Obesity Factor

Expand

Human Leptin DTSet enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Experiment Type

Species Reactivity

Detection Method

Experiment Detection Duration

Standard Curve Range

Standard Curve Gradient

Number of Incubations

Detectable Sample

Sample Loading Volume

Product Features

Kit Components

Other Required Materials

Target

UNIPROT

Synonyms

Same Target and Pathway Products

Human ULBP1/NKG2DL1 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human IL-7 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CCL3/MIP-1 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CD200R1 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human Renin DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CCL13/MCP-4 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human IL-33 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human von Willebrand Factor DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CXCL1/GRO alpha DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human IL-3 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human TREM2 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CD273/B7-DC/PD-L2 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CKMB DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human B7-H2/CD275 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human

Conjugate:

Human CD328/Siglec-7 DTSet enzyme-linked immunoassay kit

Application: ELISA

Reactivity: Human