Human Coagulation factor XII/F12, Tag Free Protein
Catalog# HA211374
Human Coagulation factor XII/F12, Tag Free Protein
Product Information
Product name
Human Coagulation factor XII/F12, Tag Free Protein
Catalog No.
HA211374
Predicted molecular mass
40.4 kD
Protein construction description
A DNA sequence encoding the human Coagulation factor XII/F12 protein (P00748) (Ile 20-Arg 372) was expressed with tag free.
Accession
P00748
Protein construction
Coagulation factor XII/F12 (20-372)
Source
HEK293
Bio Activity
Testing in progress.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Coagulation factor XII, also known as Hageman factor, is a plasma protein involved in coagulation. It is the zymogen form of factor XIIa (EC 3.4.21.38), an enzyme of the serine protease (or serine endopeptidase) class. In humans, factor XII is encoded by F12 gene. Factor XII (FXII, Hageman factor) is a plasma glycoprotein of approximately 90 kDa and is part of the coagulation cascade. It activates factor XI and prekallikrein in vitro. FXII is activated to FXIIa by negatively charged surfaces such as glass, initiating the intrinsic pathway. FXII also plays a role in diagnostic coagulation assays called activated partial thromboplastin times (aPTT).
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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