Human PDGF-B, Tag Free Protein
Catalog# HA210859
Human PDGF-B, Tag Free Protein
Product Information
Product name
Human PDGF-B, Tag Free Protein
Catalog No.
HA210859
Predicted molecular mass
12.7 kD
Protein construction description
A DNA sequence encoding the human PDGF-B protein (P01127-1) (Ser 82-Thr 190) was expressed with tag free.
Accession
P01127-1
Protein construction
PDGF-B (82-190)
Source
HEK293
Bio Activity
Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 25-100 ng/mL.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha (PDGFA) polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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