Human FGF-8b, Tag Free Protein
Active
Catalog# HA211414
Human FGF-8b, Tag Free Protein
Product Information
Product name
Human FGF-8b, Tag Free Protein
Catalog No.
HA211414
Predicted molecular mass
24.4 kD
Protein construction description
A DNA sequence encoding the human FGF-8b protein (P55075-3) (Gln 23-Arg 215) was expressed with tag Free.
Accession
P55075-3
Protein construction
FGF-8b (23-215)
Source
HEK293
Bio Activity
Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 10-60 ng/mL.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Fibroblast growth factor 8 (FGF-8) is a protein that in humans is encoded by the FGF8 gene. The protein encoded by this gene belongs to the fibroblast growth factor (FGF) family. FGF proteins are multifunctional signaling molecules with broad mitogenic and cell survival activity, playing critical roles in embryonic development, cell proliferation, morphogenesis, tissue repair, and tumor progression. FGF8 signals primarily through fibroblast growth factor receptor 1 (FGFR1) to trigger downstream pathways involved in neural and limb development.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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