Human 14-3-3 gamma/YWHAG, Tag Free Protein

Usd: 129

Specification

20μg

50μg

100μg

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Catalog# HA211350

Human 14-3-3 gamma/YWHAG, Tag Free Protein

Product Information

Product name

Human 14-3-3 gamma/YWHAG, Tag Free Protein

Catalog No.

HA211350

Predicted molecular mass

29.1 kD

Protein construction description

A DNA sequence encoding the human 14-3-3 gamma/YWHAG protein (P61981) (Met 1-Asn 247) was expressed with tag free.

Accession

P61981

Protein construction

14-3-3 gamma/YWHAG (1-247)

Source

HEK293

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

Human 14-3-3 gamma/YWHAG, Tag Free Protein

Usd: 129

Specification

Product Information

Product name

Catalog No.

Predicted molecular mass

Protein construction description

Accession

Protein construction

Source

Bio Activity

Purity

Endotoxin

Formulation

Species

Background

Usage guide

Shipping

Storage

Reconstitution

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