Human Apolipoprotein E, Tag Free
Catalog# HA211078
Human Apolipoprotein E, Tag Free
Product Information
Product name
Human Apolipoprotein E, Tag Free
Catalog No.
HA211078
Predicted molecular mass
35 kD
Protein construction description
A DNA sequence encoding the human Apolipoprotein E protein (P02649) (Lys 19-His 317) was expressed with tag free.
Accession
P02649
Protein construction
Source
HEK293
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
Products with the same target and pathway
Human IL1RL1/DER4/ST2, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human ATF6, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human NFL, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL-6, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Alpha-fetoprotein, C-His Tag
Application:
Reactivity: Human
Conjugate:
Human VEGF-C, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Antithrombin III, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TNF RI/TNFRSF1A, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CXCL10, C-Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human PMCA1/ATP2B1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human SAA1, C-Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Caspase-9/CASP9, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CNTF, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human EPCR/PROCR/CD201, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human DVL2, C Twin Strep, Flag Protein
Application:
Reactivity: Human
Conjugate:
Human ACE2, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human SPR, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-18, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Sorcin/SRI, N-Twin Strep, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human EIF2AK3/PERK, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-255), C His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-33, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TNF-alpha, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Lipocalin-2/NGAL, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human GHRL, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Syndecan-1/CD138, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human FGF Basic/FGF2, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL1RL1/DER4/ST2, C-hFc, His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human Clusterin, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human E-Selectin/CD62E, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human Fas/TNFRSF6/CD95, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human UCH-L1/PGP9.5, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL-17F, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human KIF5B, N-Twin Strep, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Angiogenin, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Enolase 2/ENO2, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CNTF, N-His Tag, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human VEGF165, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CALCA, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human HGF, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human M-CSF/CSF1 (33-190), Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human C-Reactive Protein/CRP, C-His, Flag Tag
Application:
Reactivity: Human
Conjugate:
Human IL-8/CXCL8 Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CKMB heterodimer, C-Flag, StrepII Tag&C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Apolipoprotein E/APOE4 (C130R), Tag Free
Application:
Reactivity: Human
Conjugate:
Human HGF, C-Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Cystatin C, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human tPA, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TNF-alpha, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human DKK1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human NG2/MCSP/CSPG4, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human NRG1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Transthyretin/TTR, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Haptoglobin/HP, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human DLK1, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CASQ1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human ANXA6, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Siglec-7/CD328, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human RBP4, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human G-CSF, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IMMT/Mitofilin, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CASQ2, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human VSNL1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-1 beta/IL1B, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human FGF2/b-FGF, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human NR4A2/NURR1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CKM, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human ICAM-1/CD54, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human PDGF-B, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human VEGF121, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human TNF RII/TNFRSF1B, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human GFAP, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Annexin A6/ANXA6, N-Twin Strep, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IP-10/CXCL10, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human PPID, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Leptin, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL-1 alpha/IL1A, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Periostin/OSF-2, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human NRG1, C-Flag (176-246) Protein
Application:
Reactivity: Human
Conjugate:
Human CCL2/MCP1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Adiponectin/Acrp30, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Beta-2-microglobulin/B2M, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Tau/Tau441, C-His (isoform 2N4R) Protein
Application:
Reactivity: Human
Conjugate:
Human VE-cadherin, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human LILRB4/ILT3, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human COX5A, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-6, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TIE2/TEK, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human Pentraxin 3/PTX3, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human A2M/alpha-2-macroglobulin, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human NCAM1/CD56, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human E-Cadherin, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human Resistin, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human VEGFR2/KDR, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
