Human IL-1 alpha/IL1A, C-His, Flag Tag Protein

Usd: 159 Special Discount

Specification

20μg

50μg

100μg

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Catalog# HA210602

Human IL-1 alpha/IL1A, C-His, Flag Tag Protein

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Product Information

Product name

Human IL-1 alpha/IL1A, C-His, Flag Tag Protein

Catalog No.

HA210602

Predicted molecular mass

20.7 kD

Protein construction description

A DNA sequence encoding the human IL-1 alpha/IL1A protein (P01583) (Ser 113-Ala 271) was expressed with both His, Flag tag at the C-terminus.

Accession

P01583

Protein construction

IL-1 alpha/IL1A (113-271) His Flag

Source

HEK293

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.