Human IL-10, C-His Tag Protein
Catalog# HA210729
Human IL-10, C-His Tag Protein
Product Information
Product name
Human IL-10, C-His Tag Protein
Catalog No.
HA210729
Predicted molecular mass
20 kD
Protein construction description
A DNA sequence encoding the human IL-10 protein (P22301) (Ser 19-Asn 178) was expressed with a His tag at the C-terminus.
Accession
P22301
Protein construction
IL-10 (19-178)
His
Source
HEK293
Bio Activity
Testing in progress.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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Human CXCL4/PF4, Tag Free Protein
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
Human CD200, C-His Tag (ECD) Protein
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
Human CD147/Basigin/EMMPRIN, C-His Tag Protein
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
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Human IL-3R alpha/CD123, C-His Tag Protein
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Conjugate:
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Reactivity: Human
Conjugate:
Human CCL22 / MDC, C-hFc Tag Protein
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Application:
Reactivity: Human
Conjugate:
Human IL-2RB/CD122, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IFN-gamma, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human 4-1BB/TNFRSF9, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human Siglec-7/CD328, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human G-CSF, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-1 beta/IL1B, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human EGF, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL-4, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human IL-2, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human HVEM/TNFRSF14, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human ICAM-1/CD54, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human PD-L1/B7-H1, C-hFc Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-16, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TNF RII/TNFRSF1B, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human PD-L1/B7-H1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human B7-H3, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IL-29/IFN-lambda 1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Osteoprotegerin / TNFRSF11B, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CD272/BTLA, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human HVEM/TNFRSF14, C-Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human MAPK6, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CD5, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IP-10/CXCL10, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CXCL12/SDF-1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CD27/TNFRSF7, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human LILRB1/CD85j/ILT2, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human GITR/TNFRSF18/AITR, C-Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CD2, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IL-1 alpha/IL1A, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-4R, C-His, Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CXCL9, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CCL2/MCP1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human CTLA-4, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CD31/PECAM-1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human P-Selectin/CD62P, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IL12B/P40, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human PD-1, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human VCAM-1 / CD106, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human VE-cadherin, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human LILRB4/ILT3, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IL-18 BPa, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CD63, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CD207, C-Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human ANPEP/CD13, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CXCL1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human B7-DC/PD-L2/CD273, C-Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IL-6, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL-6R, C-His (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human GADD45A, N-Twin Strep, C-His, Flag Tag Protein
Application:
Reactivity: Human
Conjugate:
Human TIE2/TEK, C-His, Flag Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human NCAM1/CD56, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human DPP-4/CD26/DPPIV, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human CD14, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human CYLD, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human IL2RG/CD132, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human E-Cadherin, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human TIMP1, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human VEGFR2/KDR, Tag Free (ECD) Protein
Application:
Reactivity: Human
Conjugate:
Human IFN-gamma R1, C-His Tag (ECD) Protein
Application:
Reactivity: Human
Conjugate:
