Human IL-10, Tag Free Protein
Active
Catalog# HA210988
Human IL-10, Tag Free Protein
Product Information
Product name
Human IL-10, Tag Free Protein
Catalog No.
HA210988
Predicted molecular mass
18.7 kD
Protein construction description
A DNA sequence encoding the human IL-10 protein (P22301) (Ser 19-Asn 178) was expressed with tag free.
Accession
P22301
Protein construction
IL-10 (19-178)
Source
HEK293
Bio Activity
Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is 0.18-2 ng/mL.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Application:
Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
Conjugate:
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Reactivity: Human
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Reactivity: Human
Conjugate:
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Reactivity: Human
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Reactivity: Human
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