Mouse IL-27 P28/IL-30 enzyme-linked immunoassay kit(one step)
Catalog# EM0051
Mouse IL-27 P28/IL-30 enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
6.86~5000 pg/ml
Overview
Product Name
Mouse IL-27 P28/IL-30 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
2.88 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0161 | 0.0134 | 0.0148 | |
| 6.86 | 0.0262 | 0.0250 | 0.0256 | 0.0109 |
| 20.58 | 0.0513 | 0.0523 | 0.0518 | 0.0371 |
| 61.73 | 0.1410 | 0.1358 | 0.1384 | 0.1237 |
| 185.19 | 0.4089 | 0.3603 | 0.3846 | 0.3699 |
| 555.56 | 1.2344 | 1.1862 | 1.2103 | 1.1956 |
| 1666.67 | 3.3594 | 3.0607 | 3.2101 | 3.1953 |
| 5000.00 | 4.6883 | 4.6819 | 4.6851 | 4.6704 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 120.21 | 516.65 | 1814.00 | 120.21 | 516.65 | 1814.00 |
| Standard Deviation | 4.48 | 18.59 | 93.98 | 4.48 | 18.59 | 93.98 |
| Coefficient of Variation (%) | 3.7 | 3.6 | 5.2 | 3.7 | 3.6 | 5.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠IL-27 P28，未加小鼠的IL-27 P28血清作为本底，计算回收率。回收率的范围从 94 % 至 102%，平均回收率为 99%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.05-888.37 | 100 | 96.1 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Function
IL-27是一种异二聚体细胞激素，由EBI3（与IL-12和IL-23的p40亚基相关）和p28（与IL-12的p35链相关）组成。IL-27由单核细胞、内皮细胞和树突状细胞表达，通过由IL-27 R α/WSX-1/TCCR和gp130组成的受体复合物传递信号。IL-27诱导IL-12受体在未发育的CD4 T细胞上表达，使其对后续IL-12诱导的Th1发育产生产生敏感。它还通过限制T和NKT细胞活性，发挥抗炎细胞因子的作用。