Mouse Resistin enzyme-linked immunoassay kit
Catalog# EMY0063
Mouse Resistin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
1.56~100 ng/ml
Overview
Product Name
Mouse Resistin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.001 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0286 | 0.0296 | 0.0291 | |
| 1.56 | 0.0844 | 0.0949 | 0.0897 | 0.0606 |
| 3.13 | 0.1841 | 0.1639 | 0.1740 | 0.1449 |
| 6.25 | 0.3404 | 0.3503 | 0.3454 | 0.3163 |
| 12.50 | 0.6524 | 0.5893 | 0.6209 | 0.5918 |
| 25.00 | 1.1861 | 1.1035 | 1.1448 | 1.1157 |
| 50.00 | 2.3761 | 2.4024 | 2.3893 | 2.3602 |
| 100.00 | 3.9695 | 4.1788 | 4.0742 | 4.0451 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 10.5 | 28.4 | 1.9 | 9.3 | 28.6 |
| Standard Deviation | 0.1 | 0.7 | 1.6 | 0.1 | 0.6 | 1.0 |
| Coefficient of Variation (%) | 6.1 | 6.4 | 5.8 | 5.5 | 6.0 | 3.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠Resistin，未加小鼠的Resistin血清作为本底，计算回收率。回收率的范围从 88 % 至 105%，平均回收率为 97%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.23-18.70 | 100 | 11.5 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
Function
抵抗素，也称为ADSF和FIZZ3，是一种分泌肽激素，作为非共价三聚体或二硫键连接的同六聚体循环。抵抗蛋白由白色脂肪细胞、脾细胞、星形胶质细胞和前垂体上皮表达。它阻断脂肪细胞对胰岛素刺激的葡萄糖摄取，并促进肝细胞的葡萄糖释放。高循环胰岛素水平会诱导抵抗蛋白分泌，进而抑制胰岛素的作用。