Mouse Leptin enzyme-linked immunoassay kit
Catalog# EM0027
Mouse Leptin enzyme-linked immunoassay kit
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sELISA
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Mouse
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13.72~10000 pg/ml
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unconjugated
Overview
Product Name
Mouse Leptin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.24 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0170 | 0.0179 | 0.0175 | |
| 13.72 | 0.0655 | 0.0633 | 0.0644 | 0.0470 |
| 41.15 | 0.1510 | 0.1464 | 0.1487 | 0.1313 |
| 123.46 | 0.3596 | 0.3765 | 0.3681 | 0.3506 |
| 370.37 | 0.9335 | 0.9369 | 0.9352 | 0.9178 |
| 1111.11 | 2.0700 | 2.0207 | 2.0454 | 2.0279 |
| 3333.33 | 3.2890 | 3.2855 | 3.2873 | 3.2698 |
| 10000.00 | 3.8812 | 4.0509 | 3.9661 | 3.9486 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 180.9 | 1004.4 | 3082.4 | 174.2 | 1023.2 | 3039.1 |
| Standard Deviation | 7.4 | 50.0 | 202.9 | 6.5 | 53.0 | 190.8 |
| Coefficient of Variation (%) | 4.1 | 5.0 | 6.6 | 3.8 | 5.2 | 6.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Leptin into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 103% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1419.67-3710.99 | 100 | 4633.39 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Leptin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
FLJ94114
LEP
LEP_HUMAN
LEPD
Leptin (murine obesity homolog)
Leptin (obesity homolog
mouse)
Leptin
Leptin Murine Obesity Homolog
Leptin Precursor Obesity Factor
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