Mouse IL-33 enzyme-linked immunoassay kit
Catalog# EM0033
Mouse IL-33 enzyme-linked immunoassay kit
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ELISA
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Mouse
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6.86~5000 pg/ml
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unconjugated
Overview
Product Name
Mouse IL-33 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.33 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0176 | 0.0174 | 0.0175 | |
| 6.86 | 0.0225 | 0.0230 | 0.0228 | 0.0053 |
| 20.58 | 0.0342 | 0.0350 | 0.0346 | 0.0171 |
| 61.73 | 0.0675 | 0.0710 | 0.0693 | 0.0518 |
| 185.19 | 0.1737 | 0.1767 | 0.1752 | 0.1577 |
| 555.56 | 0.5058 | 0.5463 | 0.5261 | 0.5086 |
| 1666.67 | 1.3030 | 1.4530 | 1.3780 | 1.3605 |
| 5000.00 | 3.0570 | 3.1460 | 3.1015 | 3.0840 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 117.2 | 525.0 | 1698.4 | 89.6 | 465.7 | 1468.5 |
| Standard Deviation | 5.8 | 33.7 | 75.0 | 2.8 | 22.1 | 69,2 |
| Coefficient of Variation (%) | 5.0 | 6.4 | 4.4 | 3.2 | 4.7 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-33 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 107% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.55 | 53 | 0.53 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-33 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C9orf26
CHROMOSOME 9 OPEN READING FRAME 26
DKFZp586H0523
DVS27
DVS27 related protein
IL 1F11
IL 33
IL-1F11
IL-33
IL1F11
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