Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
Catalog# EM0031
Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Mouse CCL3/MIP-1 alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.51 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0159 | 0.0141 | 0.0150 | |
| 1.37 | 0.0232 | 0.0210 | 0.0221 | 0.0071 |
| 4.12 | 0.0341 | 0.0302 | 0.0322 | 0.0172 |
| 12.35 | 0.0651 | 0.0658 | 0.0655 | 0.0505 |
| 37.04 | 0.1703 | 0.1707 | 0.1705 | 0.1555 |
| 111.11 | 0.4507 | 0.4318 | 0.4413 | 0.4263 |
| 333.33 | 1.2730 | 1.2200 | 1.2465 | 1.2315 |
| 1000.00 | 3.1870 | 3.2800 | 3.2335 | 3.2185 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.8 | 88.6 | 247.5 | 18.5 | 96.2 | 282.2 |
| Standard Deviation | 1.4 | 3.9 | 13.2 | 1.2 | 6.7 | 10.1 |
| Coefficient of Variation (%) | 7.3 | 4.4 | 5.3 | 6.6 | 6.9 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CCL3 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 106% to 139% with an overall mean recovery of 128%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.2-20.8 | 100 | 4.19 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CCL5 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C C motif chemokine 3
CCL 3
CCL3
CCL3_HUMAN
Chemokine C C motif ligand 3
G0/G1 switch regulatory protein 19 1
G0/G1 switch regulatory protein 19-1
G0S19 1
G0S19 1 protein
Heparin binding chemotaxis protein
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