Mouse TREM2 enzyme-linked immunoassay kit
Catalog# EM0039
Mouse TREM2 enzyme-linked immunoassay kit
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ELISA
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Mouse
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2.74~2000 pg/ml
Overview
Product Name
Mouse TREM2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.60 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0253 | 0.0312 | 0.0283 | |
| 2.74 | 0.0348 | 0.0328 | 0.0338 | 0.0056 |
| 8.23 | 0.0466 | 0.0418 | 0.0442 | 0.0160 |
| 24.69 | 0.0882 | 0.0877 | 0.0880 | 0.0597 |
| 74.07 | 0.1885 | 0.1928 | 0.1907 | 0.1624 |
| 222.22 | 0.5228 | 0.4881 | 0.5055 | 0.4772 |
| 666.67 | 1.3710 | 1.3660 | 1.3685 | 1.3403 |
| 2000.00 | 3.1040 | 3.1460 | 3.1250 | 3.0968 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 35.4 | 192.9 | 626.2 | 39.0 | 202.6 | 679.0 |
| Standard Deviation | 2.7 | 13.0 | 47.8 | 1.8 | 7.6 | 34.7 |
| Coefficient of Variation (%) | 7.6 | 6.7 | 7.6 | 4.5 | 3.8 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse TREM2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 107% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 7959.19-21290.31 | 100 | 14637.59 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
TREM 2
TREM-2
TREM2
TREM2_HUMAN
TREM2a
TREM2b
TREM2c
Trggering receptor expressed on myeloid cells 2
Trggering receptor expressed on myeloid cells 2a
Triggering receptor expressed on monocytes 2
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