Mouse CCL2/MCP-1 enzyme-linked immunoassay kit
Catalog# EM0025
Mouse CCL2/MCP-1 enzyme-linked immunoassay kit
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ELISA
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Mouse
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6.86~5000 pg/ml
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unconjugated
Overview
Product Name
Mouse CCL2/MCP-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.78 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0074 | 0.0068 | 0.0071 | |
| 6.86 | 0.0115 | 0.0114 | 0.0115 | 0.0044 |
| 20.58 | 0.0150 | 0.0166 | 0.0158 | 0.0087 |
| 61.73 | 0.0318 | 0.0326 | 0.0322 | 0.0251 |
| 185.19 | 0.0893 | 0.0986 | 0.0940 | 0.0869 |
| 555.56 | 0.2921 | 0.3009 | 0.2965 | 0.2894 |
| 1666.67 | 1.1280 | 1.1620 | 1.1450 | 1.1379 |
| 5000.00 | 3.5970 | 3.5747 | 3.5859 | 3.5788 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 122.5 | 532.2 | 1655.9 | 135.6 | 526.3 | 1598.4 |
| Standard Deviation | 9.1 | 23.5 | 68.1 | 8.4 | 24.1 | 60.7 |
| Coefficient of Variation (%) | 7.4 | 4.4 | 4.1 | 6.2 | 4.6 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CCL2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 119% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.88-82.71 | 100 | 51.74 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CCL2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-C motif chemokine 2
CCL2
CCL2_HUMAN
Chemokine (C C motif) ligand 2
GDCF 2
GDCF-2
GDCF2
HC11
HSMCR30
JE
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