Mouse IFN-γ enzyme-linked immunoassay kit
Catalog# EM0009
Mouse IFN-γ enzyme-linked immunoassay kit
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ELISA
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Mouse
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40.96~10000 pg/ml
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unconjugated
Overview
Product Name
Mouse IFN-γ enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.24 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0258 | 0.0239 | 0.0249 | |
| 40.96 | 0.0366 | 0.0323 | 0.0345 | 0.0096 |
| 102.40 | 0.0521 | 0.0522 | 0.0522 | 0.0273 |
| 256.00 | 0.0948 | 0.0997 | 0.0973 | 0.0724 |
| 640.00 | 0.2249 | 0.2222 | 0.2236 | 0.1987 |
| 1600.00 | 0.5414 | 0.5407 | 0.5411 | 0.5162 |
| 4000.00 | 1.2250 | 1.2290 | 1.2270 | 1.2022 |
| 10000.00 | 2.8770 | 2.7990 | 2.8380 | 2.8132 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 158.3 | 1074.0 | 2844.2 | 167.9 | 1116.9 | 2911.3 |
| Standard Deviation | 7.3 | 59.9 | 139.7 | 8.8 | 58.2 | 158.0 |
| Coefficient of Variation (%) | 4.6 | 5.6 | 4.9 | 5.2 | 5.2 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IFN-γ into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 121% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d. | 0 | n.d. |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IFN-γ in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Mouse | See 2 publications below |
Target
UNIPROT
Alias
Function
Citation
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Single-cell transcriptomics unveils the immunologic landscape of anti-PD-1-associated indirect drug-induced liver injury
Journal: Journal of Gastroenterology
DOI: 10.1007/s00535-026-02370-z
IF: 5.5
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb
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Intranasal Vaccination with a Recombinant Adeno-Associated Virus Type 6 Encoding SapM Confers Protection Against Tuberculosis
Journal: Vaccines
DOI: 10.3390/vaccines14030224
IF: 3.4
Application: ELISA
Reactivity: Mouse
Publish date: 2026 Feb