Human CNTF enzyme-linked immunoassay kit
Catalog# EH0147
Human CNTF enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
2.74~2000 pg/ml
Overview
Product Name
Human CNTF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.25 pg/ml (50 μl)；3.77 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0328 | 0.0323 | 0.0326 | |
| 2.74 | 0.0495 | 0.0492 | 0.0494 | 0.0168 |
| 8.23 | 0.0814 | 0.0796 | 0.0805 | 0.0480 |
| 24.69 | 0.1741 | 0.1868 | 0.1805 | 0.1479 |
| 74.07 | 0.4648 | 0.4985 | 0.4817 | 0.4491 |
| 222.22 | 1.2410 | 1.3150 | 1.2780 | 1.2455 |
| 666.67 | 3.0640 | 3.2330 | 3.1485 | 3.1160 |
| 2000.00 | 4.5481 | 4.6198 | 4.5840 | 4.5514 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 39.2 | 197.9 | 618.1 | 37.0 | 199.0 | 601.7 |
| Standard Deviation | 1.7 | 8.3 | 30.7 | 2.2 | 14.8 | 39.7 |
| Coefficient of Variation (%) | 4.4 | 4.2 | 5.0 | 6.0 | 7.4 | 6.6 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-81.17 | 13 | 42.58 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Ciliary neurotrophic factor
CNTF
CNTF_HUMAN
HCNTF