Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
Catalog# EH0209
Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.15 pg/ml (50 μl)；0.56 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0087 | 0.0088 | |
| 1.37 | 0.0154 | 0.0144 | 0.0149 | 0.0061 |
| 4.12 | 0.0311 | 0.0299 | 0.0305 | 0.0217 |
| 12.35 | 0.0837 | 0.0752 | 0.0795 | 0.0707 |
| 37.04 | 0.2048 | 0.2172 | 0.2110 | 0.2022 |
| 111.11 | 0.5411 | 0.5202 | 0.5307 | 0.5219 |
| 333.33 | 1.6650 | 1.5420 | 1.6035 | 1.5947 |
| 1000.00 | 3.3770 | 3.2140 | 3.2955 | 3.2867 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 30.3 | 151.2 | 497.0 | 23.7 | 115.1 | 364.0 |
| Standard Deviation | 1.9 | 9.7 | 37.0 | 1.3 | 3.5 | 7.8 |
| Coefficient of Variation (%) | 6.2 | 6.4 | 7.4 | 5.6 | 3.0 | 2.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IL-29/IFN-Iambda 1 ，未加人 IL-29/IFN-Iambda 1 的血清作为本底，计算回收率。回收率的范围从 87 % 至 110 %，平均回收率为 96%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-3.18 | 81.3 | 0.78 |
应用本试剂盒，检测30份健康人和5份健康狗的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Cytokine ZCYTO21
IFN lambda 1
IFN-lambda-1
IFNL1
IL-29
IL29
IL29_HUMAN
Interferon lambda 1
Interferon lambda-1
Interleukin 29 (interferon lambda 1)
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