Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
Catalog# EH0209
Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human/Canine IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.15 pg/ml (50 μl)；0.56 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0087 | 0.0088 | |
| 1.37 | 0.0154 | 0.0144 | 0.0149 | 0.0061 |
| 4.12 | 0.0311 | 0.0299 | 0.0305 | 0.0217 |
| 12.35 | 0.0837 | 0.0752 | 0.0795 | 0.0707 |
| 37.04 | 0.2048 | 0.2172 | 0.2110 | 0.2022 |
| 111.11 | 0.5411 | 0.5202 | 0.5307 | 0.5219 |
| 333.33 | 1.6650 | 1.5420 | 1.6035 | 1.5947 |
| 1000.00 | 3.3770 | 3.2140 | 3.2955 | 3.2867 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 30.3 | 151.2 | 497.0 | 23.7 | 115.1 | 364.0 |
| Standard Deviation | 1.9 | 9.7 | 37.0 | 1.3 | 3.5 | 7.8 |
| Coefficient of Variation (%) | 6.2 | 6.4 | 7.4 | 5.6 | 3.0 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human IL-29/IFN-Iambda 1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 110% with an overall mean recovery of 96%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-3.18 | 81.3 | 0.78 |
| Canine Serum | 5 | n.d.-17.29 | 80 | 13.21 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and five samples from healthy canines swere evaluated for the presence of IL-29/IFN-Iambda 1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Cytokine ZCYTO21
IFN lambda 1
IFN-lambda-1
IFNL1
IL-29
IL29
IL29_HUMAN
Interferon lambda 1
Interferon lambda-1
Interleukin 29 (interferon lambda 1)
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