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Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit (EH0177)

Price: Regular price $499.00

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Catalog# EH0177

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.09 pg/ml (50 μl)；0.15 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.06250.06110.0618
2.740.09460.10140.09800.0362
8.230.14040.13660.13850.0767
24.690.20810.19790.20300.1412
74.070.37900.37370.37640.3146
222.220.98610.99350.98980.9280
666.672.13002.17102.15052.0887
2000.003.64703.77703.71203.6502

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)37.9190.5584.142.1208.8611.7
Standard Deviation2.812.137.91.914.541.6
Coefficient of Variation(%)7.46.36.55.34.24.6

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum306.34-126.1810033.78

Sensitivity

0.09 pg/ml (50 μl)；0.15 pg/ml (10 μl)；

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-1RA into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 109% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit (EH0177)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P18510

PROTEIN NAME

IL-1RA/IL-1F3

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