Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit
Catalog# EH0177
Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
2.74~2000 pg/ml
Overview
Product Name
Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.09 pg/ml (50 μl)；0.15 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0625 | 0.0611 | 0.0618 | |
| 2.74 | 0.0946 | 0.1014 | 0.0980 | 0.0362 |
| 8.23 | 0.1404 | 0.1366 | 0.1385 | 0.0767 |
| 24.69 | 0.2081 | 0.1979 | 0.2030 | 0.1412 |
| 74.07 | 0.3790 | 0.3737 | 0.3764 | 0.3146 |
| 222.22 | 0.9861 | 0.9935 | 0.9898 | 0.9280 |
| 666.67 | 2.1300 | 2.1710 | 2.1505 | 2.0887 |
| 2000.00 | 3.6470 | 3.7770 | 3.7120 | 3.6502 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 37.9 | 190.5 | 584.1 | 42.1 | 208.8 | 611.7 |
| Standard Deviation | 2.8 | 12.1 | 37.9 | 1.9 | 14.5 | 41.6 |
| Coefficient of Variation (%) | 7.4 | 6.3 | 6.5 | 5.3 | 4.2 | 4.6 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 6.34-126.18 | 100 | 33.78 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃