Human CD80 enzyme-linked immunoassay kit
Catalog# EH0121
Human CD80 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 pg/ml
Overview
Product Name
Human CD80 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.08 pg/ml (50 μl)；0.52 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0107 | 0.0107 | 0.0107 | |
| 1.37 | 0.0294 | 0.0283 | 0.0289 | 0.0182 |
| 4.12 | 0.0649 | 0.0648 | 0.0649 | 0.0542 |
| 12.35 | 0.1801 | 0.1761 | 0.1781 | 0.1674 |
| 37.04 | 0.5149 | 0.5114 | 0.5132 | 0.5025 |
| 111.11 | 1.3710 | 1.4240 | 1.3975 | 1.3868 |
| 333.33 | 3.0950 | 3.2490 | 3.1720 | 3.1613 |
| 1000.00 | 4.2695 | 4.3810 | 4.3253 | 4.3146 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.9 | 106.6 | 336.5 | 22.2 | 109.1 | 333.8 |
| Standard Deviation | 1.2 | 7.2 | 32.8 | 0.7 | 3.4 | 11.9 |
| Coefficient of Variation (%) | 3.9 | 6.8 | 5.9 | 3.0 | 3.2 | 3.6 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 17.39-112.32 | 100 | 70.39 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Activation B7-1 antigen
B lymphocyte activation antigen B7
B7
B7-1
B7-1 antigen
B7.1
BB1
CD28 antigen ligand 1
CD28LG
CD28LG1
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