Human CTLA-4 enzyme-linked immunoassay kit
Catalog# EH0179
Human CTLA-4 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
2.74~2000 pg/ml
Overview
Product Name
Human CTLA-4 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.33 pg/ml (50 μl)；1.97 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0105 | 0.0103 | 0.0104 | |
| 2.74 | 0.0171 | 0.0159 | 0.0165 | 0.0061 |
| 8.23 | 0.0282 | 0.0271 | 0.0277 | 0.0173 |
| 24.69 | 0.0616 | 0.0611 | 0.0614 | 0.0510 |
| 74.07 | 0.1683 | 0.1480 | 0.1582 | 0.1478 |
| 222.22 | 0.5143 | 0.4790 | 0.4967 | 0.4863 |
| 666.67 | 1.5540 | 1.5790 | 1.5665 | 1.5561 |
| 2000.00 | 3.9590 | 3.9100 | 3.9345 | 3.9241 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 53.1 | 266.2 | 814.9 | 17.2 | 77.5 | 231.4 |
| Standard Deviation | 2.9 | 9.1 | 34.8 | 1.3 | 5.1 | 12.0 |
| Coefficient of Variation (%) | 5.5 | 3.4 | 4.3 | 7.8 | 6.6 | 5.2 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.31-3.78 | 100 | 1.11 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃