Human PD-1 enzyme-linked immunoassay kit
Catalog# EH0062
Human PD-1 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 pg/ml
Overview
Product Name
Human PD-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.05 pg/ml (50 μl)；1.64 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0143 | 0.0147 | |
| 1.37 | 0.0296 | 0.0294 | 0.0295 | 0.0149 |
| 4.12 | 0.0578 | 0.0573 | 0.0576 | 0.0429 |
| 12.35 | 0.1454 | 0.1387 | 0.1421 | 0.1274 |
| 37.04 | 0.3980 | 0.3963 | 0.3972 | 0.3825 |
| 111.11 | 1.1110 | 1.1360 | 1.1235 | 1.1089 |
| 333.33 | 2.6150 | 2.5850 | 2.6000 | 2.5854 |
| 1000.00 | 4.1695 | 4.1661 | 4.1678 | 4.1532 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 29.9 | 126.7 | 422.8 | 25.1 | 108.9 | 326.8 |
| Standard Deviation | 1.6 | 6.3 | 25.8 | 1.8 | 6.3 | 16.2 |
| Coefficient of Variation (%) | 5.3 | 5.0 | 6.1 | 7.2 | 5.8 | 4.9 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 90.86-174.38 | 100 | 130.26 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃