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Human uPAR enzyme-linked immunoassay kit (EH0077)

Price: Regular price $499.00

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Catalog# EH0077

Assay type Sandwich ELISA

Standard curve range 27.43~20000 pg/ml

Sensitivity 8.79 pg/ml (50 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01480.01660.0157
27.430.02620.02380.02500.0093
82.300.04530.04910.04720.0315
246.910.10610.10350.10480.0891
740.740.27010.27230.27120.2555
2222.220.79350.77770.78560.7699
6666.672.04102.02802.03452.0188
20000.003.91803.90103.90953.8938

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)370.11850.16148.8356.91784.46135.0
Standard Deviation10.287.9204.118.488.2389.3
Coefficient of Variation(%)2.84.83.35.24.96.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301694.27-8807.191003895.95

Sensitivity

8.79 pg/ml (50 μl)

Standard curve range

27.43~20000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human uPAR into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 103% with an overall mean recovery of 96%.

Specifications

Product Type

ELISA Kit

Product Name

Human uPAR enzyme-linked immunoassay kit (EH0077)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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