Human IFN-α enzyme-linked immunoassay kit
Catalog# EH0085
Human IFN-α enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.41~300 pg/ml
Overview
Product Name
Human IFN-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0187 | 0.0181 | 0.0184 | |
| 0.41 | 0.0407 | 0.0408 | 0.0408 | 0.0224 |
| 1.23 | 0.0767 | 0.0753 | 0.0760 | 0.0576 |
| 3.70 | 0.1796 | 0.1758 | 0.1777 | 0.1593 |
| 11.11 | 0.4858 | 0.4840 | 0.4849 | 0.4665 |
| 33.33 | 1.2260 | 1.2340 | 1.2300 | 1.2116 |
| 100.00 | 2.5610 | 2.5950 | 2.5780 | 2.5596 |
| 300.00 | 4.2605 | 4.2605 | 4.2605 | 4.2421 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 11.4 | 51.2 | 155.6 | 12.0 | 57.0 | 175 |
| Standard Deviation | 0.5 | 3.8 | 9.8 | 0.7 | 1.8 | 4.2 |
| Coefficient of Variation (%) | 4.4 | 7.3 | 6.3 | 5.3 | 3.1 | 2.4 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.9 | 31.3 | 0.21 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IFL
IFN alpha
IFN alpha 1/13
IFN alpha 1b
IFN alphaD
IFN
IFN-alpha-1/13
IFNA@
IFNA1
IFNA1_HUMAN
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