Human IL-27 enzyme-linked immunoassay kit
Catalog# EH0094
Human IL-27 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
13.72~10000 pg/ml
Overview
Product Name
Human IL-27 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
5.51 pg/ml (50 μl)；17.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0348 | 0.0396 | 0.0372 | |
| 13.72 | 0.0476 | 0.0455 | 0.0466 | 0.0094 |
| 41.15 | 0.0701 | 0.0681 | 0.0691 | 0.0319 |
| 123.46 | 0.1287 | 0.1349 | 0.1318 | 0.0946 |
| 370.37 | 0.3138 | 0.3228 | 0.3183 | 0.2811 |
| 1111.11 | 0.8036 | 0.8394 | 0.8215 | 0.7843 |
| 3333.33 | 2.1080 | 2.1650 | 2.1365 | 2.0993 |
| 10000.00 | 4.1420 | 4.2020 | 4.1720 | 4.1348 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 205.1 | 1064.4 | 3596.5 | 203.0 | 1017.0 | 3373.2 |
| Standard Deviation | 5.0 | 35.7 | 123.5 | 11.8 | 29.4 | 104.1 |
| Coefficient of Variation (%) | 2.4 | 3.4 | 3.4 | 5.8 | 2.9 | 3.1 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-405.59 | 75 | 68.41 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃