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Human IL-27 enzyme-linked immunoassay kit (EH0094)

Price: Regular price $499.00

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Catalog# EH0094

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 5.51 pg/ml (50 μl)；17.28 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03480.03960.0372
13.720.04760.04550.04660.0094
41.150.07010.06810.06910.0319
123.460.12870.13490.13180.0946
370.370.31380.32280.31830.2811
1111.110.80360.83940.82150.7843
3333.332.10802.16502.13652.0993
10000.004.14204.20204.17204.1348

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)205.11064.43596.5203.01017.03373.2
Standard Deviation5.035.7123.511.829.4104.1
Coefficient of Variation(%)2.43.43.45.82.93.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-405.597568.41

Sensitivity

5.51 pg/ml (50 μl)；17.28 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-27 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 102% with an overall mean recovery of 91%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-27 enzyme-linked immunoassay kit (EH0094)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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