Human CD276/B7-H3 enzyme-linked immunoassay kit
Catalog# EH0101
Human CD276/B7-H3 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
6.86~5000 pg/ml
Overview
Product Name
Human CD276/B7-H3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.34 pg/ml (50 μl)；1.64 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0245 | 0.0188 | 0.0217 | |
| 6.86 | 0.0357 | 0.0299 | 0.0328 | 0.0112 |
| 20.58 | 0.0549 | 0.0489 | 0.0519 | 0.0303 |
| 61.73 | 0.1073 | 0.1138 | 0.1106 | 0.0889 |
| 185.19 | 0.2891 | 0.2753 | 0.2822 | 0.2606 |
| 555.56 | 0.7364 | 0.7668 | 0.7516 | 0.7300 |
| 1666.67 | 1.7640 | 1.6930 | 1.7285 | 1.7069 |
| 5000.00 | 3.2980 | 3.2950 | 3.2965 | 3.2749 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 100.5 | 499.3 | 1636.1 | 93.4 | 457.0 | 1443.3 |
| Standard Deviation | 5.1 | 22.3 | 81.7 | 4.2 | 18.2 | 4.0 |
| Coefficient of Variation (%) | 5.1 | 4.5 | 5.0 | 4.5 | 4.0 | 3.5 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.04-9.65 | 100.0 | 6.68 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃