Human CD27/TNFRSF7 enzyme-linked immunoassay kit
Catalog# EH0114
Human CD27/TNFRSF7 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
13.72~10000 pg/ml
Overview
Product Name
Human CD27/TNFRSF7 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
18.37 pg/ml (50 μl)；3.98 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0211 | 0.0219 | 0.0215 | |
| 13.72 | 0.0346 | 0.0309 | 0.0328 | 0.0113 |
| 41.15 | 0.0708 | 0.0662 | 0.0685 | 0.0470 |
| 123.46 | 0.1491 | 0.1387 | 0.1439 | 0.1224 |
| 370.37 | 0.3997 | 0.3502 | 0.3750 | 0.3535 |
| 1111.11 | 1.0700 | 1.1050 | 1.0875 | 1.0660 |
| 3333.33 | 2.7800 | 2.7570 | 2.7685 | 2.7470 |
| 10000.00 | 4.4793 | 4.4118 | 4.4456 | 4.4241 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 235.3 | 1382.0 | 4675.0 | 207.7 | 1101.3 | 3437.3 |
| Standard Deviation | 16.2 | 90.3 | 227.3 | 10.3 | 68.9 | 259.5 |
| Coefficient of Variation (%) | 6.9 | 6.5 | 4.9 | 5.0 | 6.3 | 7.5 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 114.75-12084.57 | 100 | 6777.71 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 27
CD27
CD27 antigen
CD27 molecule
CD27_HUMAN
CD27L receptor
LPFS2
MGC20393
OTTHUMP00000238557
S152
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