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Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit (EH0181)

Price: Regular price $499.00

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Catalog# EH0181

Assay type Sandwich ELISA

Standard curve range 40.96~10000 pg/ml

Sensitivity 10.59 pg/ml (50 μl)；83.08 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01390.01370.0138
40.960.02700.02610.02660.0128
102.400.04950.04540.04750.0337
256.000.10060.09430.09750.0837
640.000.23080.21370.22230.2085
1600.000.52870.49550.51210.4983
4000.001.24901.15501.20201.1882
10000.002.48402.40602.44502.4312

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)326.81415.94105.8226.71146.43807.0
Standard Deviation21.786.6262.98.165.968.6
Coefficient of Variation(%)6.66.16.43.65.71.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-31.502516.64

Sensitivity

10.59 pg/ml (50 μl)；83.08 pg/ml (10 μl)；

Standard curve range

40.96~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human TNF beta/Lymphotoxin alpha into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 92% to 117% with an overall mean recovery of 105%.

Specifications

Product Type

ELISA Kit

Product Name

Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit (EH0181)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P01374

PROTEIN NAME

TNF beta/Lymphotoxin alpha

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