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Human CD28 enzyme-linked immunoassay kit (EH0183)

Price: Regular price $499.00

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Catalog# EH0183

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 1.41 pg/ml (50 μl)；57.28 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01020.00920.0097
156.250.03090.03130.03110.0214
312.500.04960.05780.05370.0440
625.000.09660.10920.10290.0932
1250.000.20400.23360.21880.2091
2500.000.51390.55970.53680.5271
5000.001.32501.50901.41701.4073
10000.003.33403.59003.46203.4523

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)203.41052.03276.1166.4957.13088.7
Standard Deviation11.537.4176.23.529.791.4
Coefficient of Variation(%)5.63.65.42.13.13.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum303.32-816.93100104.41

Sensitivity

1.41 pg/ml (50 μl)；57.28 pg/ml (10 μl)；

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CD28 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 113% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Human CD28 enzyme-linked immunoassay kit (EH0183)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P10747

PROTEIN NAME

CD28

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