Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）
Catalog# EHY0132
Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.14~100 ng/ml
Overview
Product Name
Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.10 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0077 | 0.0086 | 0.0082 | |
| 0.14 | 0.0225 | 0.0239 | 0.0232 | 0.0151 |
| 0.41 | 0.0562 | 0.0586 | 0.0574 | 0.0493 |
| 1.23 | 0.1686 | 0.1711 | 0.1699 | 0.1617 |
| 3.70 | 0.5579 | 0.5243 | 0.5411 | 0.5330 |
| 11.11 | 1.6230 | 1.5880 | 1.6055 | 1.5974 |
| 33.33 | 3.4830 | 3.5050 | 3.4940 | 3.4859 |
| 100.00 | 4.3062 | 4.3968 | 4.3515 | 4.3434 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.0 | 10.6 | 29.4 | 1.8 | 10.1 | 29.2 |
| Standard Deviation | 0.1 | 0.6 | 1.4 | 0.1 | 0.5 | 1.5 |
| Coefficient of Variation (%) | 2.7 | 5.3 | 4.9 | 5.2 | 5.4 | 5.2 |
Spike Recovery
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.48-15.01 | 100 | 9.93 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7 Point/3 Folds
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B7 dendritic cell molecule
B7-DC
B7DC
bA574F11.2
Btdc
Butyrophilin B7 DC
Butyrophilin B7-DC
Butyrophilin B7DC
CD 273
CD273
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