Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)
Catalog# EHY0156
Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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4.69~300 ng/ml
Overview
Product Name
Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.07 ng/ml (50 μl)；0.35 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0064 | 0.0066 | |
| 4.69 | 0.0893 | 0.0926 | 0.0910 | 0.0844 |
| 9.38 | 0.2235 | 0.2204 | 0.2220 | 0.2154 |
| 18.75 | 0.4926 | 0.5205 | 0.5066 | 0.5000 |
| 37.50 | 1.0290 | 1.1290 | 1.0790 | 1.0724 |
| 75.00 | 1.9120 | 2.0470 | 1.9795 | 1.9729 |
| 150.00 | 2.9800 | 3.1140 | 3.0470 | 3.0404 |
| 300.00 | 3.5890 | 3.6480 | 3.6185 | 3.6119 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 6.3 | 31.3 | 98.9 | 6.0 | 31.1 | 101.0 |
| Standard Deviation | 0.2 | 1.0 | 2.8 | 0.2 | 1.3 | 4.1 |
| Coefficient of Variation (%) | 3.6 | 3.3 | 2.9 | 3.8 | 4.2 | 4.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CTGF/CCN2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 96%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.33-36.79 | 100 | 23.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CTGF/CCN2 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CCN 2
CCN family member 2
CCN2
Connective tissue growth factor
Ctgf
CTGF_HUMAN
Hcs 24
Hcs24
Hypertrophic chondrocyte specific protein 24
Hypertrophic chondrocyte-specific gene product 24
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