Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
Catalog# EHY0157
Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.27~200 ng/ml
Overview
Product Name
Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.004 ng/ml (50 μl)；13.60 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0115 | 0.0102 | 0.0109 | |
| 0.27 | 0.0451 | 0.0421 | 0.0436 | 0.0328 |
| 0.82 | 0.1053 | 0.1027 | 0.1040 | 0.0932 |
| 2.47 | 0.2837 | 0.2744 | 0.2791 | 0.2682 |
| 7.41 | 0.7716 | 0.7461 | 0.7589 | 0.7480 |
| 22.22 | 1.8110 | 1.7790 | 1.7950 | 1.7842 |
| 66.67 | 3.2530 | 3.0140 | 3.1335 | 3.1227 |
| 200.00 | 4.1758 | 3.9680 | 4.0719 | 4.0611 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.7 | 19.0 | 65.8 | 4.0 | 13.7 | 54.5 |
| Standard Deviation | 0.3 | 1.1 | 4.0 | 0.2 | 0.7 | 3.0 |
| Coefficient of Variation (%) | 4.7 | 5.9 | 6.0 | 3.9 | 5.0 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IGFBP3/IBP3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 89%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.82-8.78 | 100 | 5.74 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IGFBP3/IBP3 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Acid stable subunit of the 140 K IGF complex
Binding protein 29
Binding protein 53
BP 53
BP53
Growth hormone dependent binding protein
IBP 3
IBP-3
IBP3
IBP3_HUMAN
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