Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
Catalog# EH0134
Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
156.25~10000 pg/ml
Overview
Product Name
Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0491 | 0.0475 | 0.0483 | |
| 156.25 | 0.0991 | 0.0987 | 0.0989 | 0.0506 |
| 312.50 | 0.1488 | 0.1489 | 0.1489 | 0.1006 |
| 625.00 | 0.2471 | 0.2517 | 0.2494 | 0.2011 |
| 1250.00 | 0.4688 | 0.4276 | 0.4482 | 0.3999 |
| 2500.00 | 0.8168 | 0.8312 | 0.8240 | 0.7757 |
| 5000.00 | 1.5140 | 1.5180 | 1.5160 | 1.4677 |
| 10000.00 | 2.7410 | 2.7560 | 2.7485 | 2.7002 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 219.2 | 1152.6 | 3499.9 | 194.9 | 1053.1 | 3264.2 |
| Standard Deviation | 12.1 | 67.3 | 170.5 | 12.8 | 58.3 | 145.9 |
| Coefficient of Variation (%) | 5.5 | 5.8 | 4.9 | 6.5 | 5.5 | 4.5 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 804.66-2639.86 | 100 | 1600.42 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD123
CD123 antigen
hIL 3Ra
hIL-3Ra
hIL3Ra
IL 3 receptor alpha SP2 isoform
IL 3R alpha
IL-3 receptor subunit alpha
IL-3R subunit alpha
IL-3R-alpha
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