Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
Catalog# EHY0138
Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
-
ELISA
-
Human
-
4.69~300 ng/ml
Overview
Product Name
Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.02 ng/ml (50 μl)；0.69 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0058 | 0.0061 | |
| 4.69 | 0.0354 | 0.0345 | 0.0350 | 0.0293 |
| 9.38 | 0.0859 | 0.0789 | 0.0824 | 0.0798 |
| 18.75 | 0.2116 | 0.1990 | 0.2053 | 0.2055 |
| 37.50 | 0.5367 | 0.5317 | 0.5342 | 0.5306 |
| 75.00 | 1.3890 | 1.3180 | 1.3535 | 1.3829 |
| 150.00 | 2.5750 | 2.7090 | 2.6420 | 2.5689 |
| 300.00 | 3.6260 | 3.6480 | 3.6370 | 3.6199 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.7 | 32.8 | 151.8 | 5.3 | 31.3 | 113.6 |
| Standard Deviation | 0.2 | 1.7 | 10.4 | 0.1 | 2.0 | 7.7 |
| Coefficient of Variation (%) | 2.7 | 5.2 | 6.8 | 2.6 | 6.4 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human von Willebrand Factor into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 126% with an overall mean recovery of 109%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 10.53-1936.87 | 100 | 1056.08 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of von Willebrand Factor in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Coagulation factor VIII
Coagulation factor VIII VWF
F8VWF
Factor VIII related antigen
von Willebrand antigen 2
von Willebrand antigen II
Von Willebrand disease
VWD
vWF
VWF_HUMAN