Human IL-5 enzyme-linked immunoassay kit
Catalog# EH0008
Human IL-5 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Human IL-5 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.15 pg/ml (50 μl) ；0.73 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0324 | 0.0303 | 0.0314 | |
| 1.37 | 0.0392 | 0.0346 | 0.0369 | 0.0056 |
| 4.12 | 0.0484 | 0.0466 | 0.0475 | 0.0162 |
| 12.35 | 0.0832 | 0.0818 | 0.0825 | 0.0512 |
| 37.04 | 0.1765 | 0.1662 | 0.1714 | 0.14 |
| 111.11 | 0.4454 | 0.4245 | 0.435 | 0.4036 |
| 333.33 | 1.1039 | 1.0797 | 1.0918 | 1.0605 |
| 1000 | 2.8645 | 2.8548 | 2.8597 | 2.8283 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.3 | 77.9 | 275.8 | 16.4 | 79.9 | 291.5 |
| Standard Deviation | 0.4 | 2.4 | 11.7 | 0.3 | 1.7 | 8.9 |
| Coefficient of Variation (%) | 2.7 | 3.1 | 4.2 | 2.1 | 2.1 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-5 into health human serum sample. The un-spiked serum was used as blank in these experiments.
The recovery ranged from 88% to 115% with an overall mean recovery of 106%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-6.72 | 21 | 2.13 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B-cell differentiation factor I
Colony stimulating factor
EDF
Eosinophil differentiation factor
IL-5
IL5
IL5_HUMAN
Interleukin 5 (colony stimulating factor, eosinophil)
Interleukin 5
Interleukin-5
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