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Human IL-12p70 enzyme-linked immunoassay kit (EH0021)

Price: Regular price $499.00

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Catalog# EH0021

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 3.42 pg/ml (50 μl)；11.63 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.04320.04230.0428
13.720.05320.05270.05300.0102
41.150.07310.06680.07000.0272
123.460.12700.12110.12410.0813
370.370.27120.26950.27040.2276
1111.110.67620.61480.64550.6027
3333.331.60951.50491.55721.5144
10000.003.27713.31123.29423.2514

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)189.2970.02776.2207.21027.93062.6
Standard Deviation9.163.3179.111.451.3150.6
Coefficient of Variation(%)4.86.56.55.55.04.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3014.8-62.41736.5

Sensitivity

3.42 pg/ml (50 μl)；11.63 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-12 p70 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 78% to 119% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-12p70 enzyme-linked immunoassay kit (EH0021)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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