Human PDGF-AA enzyme-linked immunoassay kit
Catalog# EH0058
Human PDGF-AA enzyme-linked immunoassay kit
-
sELISA
-
Human
-
0.31~20 ng/ml
-
unconjugated
Overview
Product Name
Human PDGF-AA enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.10 ng/ml (50 μl)；0.17 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.2311 | 0.2403 | 0.2357 | |
| 0.31 | 0.2942 | 0.3011 | 0.2977 | 0.0619 |
| 0.63 | 0.3271 | 0.3783 | 0.3527 | 0.1170 |
| 1.25 | 0.4328 | 0.4453 | 0.4391 | 0.2034 |
| 2.50 | 0.6121 | 0.6172 | 0.6147 | 0.3790 |
| 5.00 | 1.0300 | 1.0830 | 1.0565 | 0.8208 |
| 10.00 | 1.8060 | 1.8730 | 1.8395 | 1.6038 |
| 20.00 | 3.0540 | 3.0790 | 3.0665 | 2.8308 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.41 | 2.16 | 6.48 | 0.50 | 2.35 | 6.85 |
| Standard Deviation | 0.03 | 0.11 | 0.29 | 0.03 | 0.15 | 0.35 |
| Coefficient of Variation (%) | 6.7 | 5.2 | 4.5 | 7.0 | 6.2 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PDGF-AA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 112% with an overall mean recovery of 106%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 21.10-57.89 | 100 | 33.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of PDGF-AA in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
PDGF A
PDGF A chain
PDGF subunit A
PDGF-1
PDGF1
PDGFA
PDGFA_HUMAN
Platelet derived growth factor alpha chain
Platelet derived growth factor alpha isoform 2 preproprotein
Platelet derived growth factor alpha polypeptide
Expand