Human Fetuin A/AHSG enzyme-linked immunoassay kit
Catalog# EHY0320
Human Fetuin A/AHSG enzyme-linked immunoassay kit
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sELISA
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Human
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1.56 ~100 ng/ml
Overview
Product Name
Human Fetuin A/AHSG enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.20 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0071 | 0.0075 | 0.0073 | |
| 1.56 | 0.1288 | 0.1294 | 0.1291 | 0.1218 |
| 3.13 | 0.2524 | 0.2475 | 0.2500 | 0.2427 |
| 6.25 | 0.4626 | 0.4667 | 0.4647 | 0.4574 |
| 12.50 | 0.8483 | 0.8552 | 0.8518 | 0.8445 |
| 25.00 | 1.5650 | 1.4900 | 1.5275 | 1.5202 |
| 50.00 | 2.5080 | 2.3960 | 2.4520 | 2.4447 |
| 100.00 | 3.7100 | 3.5040 | 3.6070 | 3.5997 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 9.2 | 28.2 | 2.2 | 10.6 | 31.8 |
| Standard Deviation | 0.1 | 0.3 | 1.3 | 0.1 | 0.5 | 1.4 |
| Coefficient of Variation (%) | 3.7 | 3.8 | 4.6 | 5.5 | 4.6 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Fetuin A/AHSG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 117% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 151.45-960.89 | 100 | 407.57 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Fetuin A/AHSG in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Alpha-2-HS-glycoprotein
Alpha-2-Z-globulin
Ba-alpha-2-glycoprotein
Fetuin-A
Alpha-2-HS-glycoprotein chain A
Alpha-2-HS-glycoprotein chain B
AHSG
FETUA
PRO2743