Human Uromodulin/UMOD enzyme-linked immunoassay kit
Catalog# EHY0341
Human Uromodulin/UMOD enzyme-linked immunoassay kit
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sELISA
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Human
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7.81 ~500 ng/ml
Overview
Product Name
Human Uromodulin/UMOD enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.15 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0055 | 0.0052 | 0.0054 | |
| 7.81 | 0.0775 | 0.0790 | 0.0783 | |
| 0.0729 | 15.63 | 0.1702 | 0.1636 | 0.1669 |
| 0.1616 | 31.25 | 0.3624 | 0.3516 | 0.3570 |
| 0.3517 | 62.50 | 0.7562 | 0.7053 | 0.7308 |
| 0.7254 | 125.00 | 1.4020 | 1.3710 | 1.3865 |
| 1.3812 | 250.00 | 2.3250 | 2.4100 | 2.3675 |
| 2.3622 | 500.00 | 3.8170 | 3.7300 | 3.7735 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 14.1 | 55.7 | 143.7 | 15.1 | 59.7 | 170.9 |
| Standard Deviation | 0.7 | 2.8 | 6.7 | 0.8 | 2.3 | 8.0 |
| Coefficient of Variation (%) | 4.7 | 5.0 | 4.7 | 5.0 | 3.9 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Uromodulin/UMOD into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 112% with an overall mean recovery of 99%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 88.60-324.26 | 100 | 156.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Uromodulin/UMOD in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
ADMCKD2
FJHN
HNFJ
HNFJ1
MCKD2
medullary cystic kidney disease 2 (autosomal dominant)
Tamm Horsfall glycoprotein
Tamm Horsfall urinary glycoprotein
Tamm-Horsfall urinary glycoprotein
THGP
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