Human Complement C5 enzyme-linked immunoassay kit
Catalog# EH0108
Human Complement C5 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
2.74~2000 pg/ml
Overview
Product Name
Human Complement C5 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.05 pg/ml (50 μl)；3.14 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0191 | 0.0181 | 0.0186 | |
| 2.74 | 0.0494 | 0.0480 | 0.0487 | 0.0301 |
| 8.23 | 0.0899 | 0.0917 | 0.0908 | 0.0722 |
| 24.69 | 0.1898 | 0.1834 | 0.1866 | 0.1680 |
| 74.07 | 0.4362 | 0.4138 | 0.4250 | 0.4064 |
| 222.22 | 0.9751 | 0.9995 | 0.9873 | 0.9687 |
| 666.67 | 2.2140 | 2.1390 | 2.1765 | 2.1579 |
| 2000.00 | 3.8600 | 3.8120 | 3.8360 | 3.8174 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 17.8 | 92.6 | 287.7 | 16.6 | 85.8 | 261.2 |
| Standard Deviation | 0.7 | 4.5 | 15.2 | 0.7 | 4.8 | 16.9 |
| Coefficient of Variation (%) | 3.7 | 4.9 | 5.3 | 4.5 | 5.6 | 6.5 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.79-58.93 | 100 | 25.41 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C5
Complement C5
Complement component 5
CPAMD4