Human IL-15 enzyme-linked immunoassay kit
Catalog# EH0031
Human IL-15 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
15.63~1000 pg/ml
Overview
Product Name
Human IL-15 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.91 pg/ml (50 μl) ； 4.20 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0569 | 0.0510 | 0.0540 | |
| 15.63 | 0.0986 | 0.0931 | 0.0959 | 0.0419 |
| 31.25 | 0.1385 | 0.1383 | 0.1384 | 0.0845 |
| 62.50 | 0.2296 | 0.2298 | 0.2297 | 0.1758 |
| 125.00 | 0.4239 | 0.4303 | 0.4271 | 0.3732 |
| 250.00 | 0.8343 | 0.8272 | 0.8308 | 0.7768 |
| 500.00 | 1.5410 | 1.4950 | 1.5180 | 1.4641 |
| 1000.00 | 2.8480 | 2.7570 | 2.8025 | 2.7486 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.0 | 97.8 | 305.8 | 25.0 | 120.8 | 332.4 |
| Standard Deviation | 1.0 | 7.6 | 14.0 | 1.8 | 9.4 | 17.0 |
| Coefficient of Variation (%) | 4.2 | 4.6 | 4.9 | 4.8 | 4.7 | 5.1 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.17-4.91 | 100 | 2.5 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
L15、 IL-15、IL-15MGC9721、interleukin 15、interleukin-15