Human Granzyme B enzyme-linked immunoassay kit
Catalog# EH0052
Human Granzyme B enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
78.13~5000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human Granzyme B enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
6.62 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0354 | 0.0305 | 0.0330 | |
| 78.13 | 0.0862 | 0.0936 | 0.0899 | 0.0570 |
| 156.25 | 0.1339 | 0.1493 | 0.1416 | 0.1087 |
| 312.50 | 0.2451 | 0.2431 | 0.2441 | 0.2112 |
| 625.00 | 0.3759 | 0.3953 | 0.3856 | 0.3527 |
| 1250.00 | 0.7970 | 0.7870 | 0.7920 | 0.7591 |
| 2500.00 | 1.4500 | 1.3650 | 1.4075 | 1.3746 |
| 5000.00 | 2.4690 | 2.3120 | 2.3905 | 2.3576 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 97.1 | 448.0 | 1618.5 | 84.9 | 453.4 | 1514.2 |
| Standard Deviation | 5.1 | 17.4 | 58.5 | 3.7 | 10.0 | 31.0 |
| Coefficient of Variation (%) | 5.2 | 3.9 | 3.6 | 4.3 | 2.2 | 2.1 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-58.36 | 37.5 | 14.21 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃