Human IL-17E/IL-25 enzyme-linked immunoassay kit
Catalog# EH0066
Human IL-17E/IL-25 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
2.74~2000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human IL-17E/IL-25 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.69 pg/ml (50 μl)；2.74 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0112 | 0.0117 | 0.0115 | |
| 2.74 | 0.0180 | 0.0179 | 0.0180 | 0.0065 |
| 8.23 | 0.0321 | 0.0308 | 0.0315 | 0.0200 |
| 24.69 | 0.0710 | 0.0689 | 0.0700 | 0.0585 |
| 74.07 | 0.2000 | 0.1808 | 0.1904 | 0.1790 |
| 222.22 | 0.5739 | 0.5533 | 0.5636 | 0.5522 |
| 666.67 | 1.6250 | 1.4920 | 1.5585 | 1.5471 |
| 2000.00 | 3.6720 | 3.5570 | 3.6145 | 3.6031 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 40.3 | 180.8 | 566.4 | 38.9 | 186.5 | 589.6 |
| Standard Deviation | 2.0 | 10.2 | 57.1 | 2.3 | 8.8 | 35.4 |
| Coefficient of Variation (%) | 4.9 | 5.7 | 10.1 | 5.8 | 4.7 | 6.0 |
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-4.62 | 20 | 2.30 |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
IL 17E
IL 25
IL-17E
IL-25
IL17e
IL25
IL25_HUMAN
Interleukin 17E
Interleukin 25
Interleukin-17E
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