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Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit (EH0028)

Price: Regular price $499.00

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Catalog# EH0028

Assay type Sandwich ELISA

Standard curve range 10.97~8000 pg/ml

Sensitivity 8.58 pg/ml (50 μl)；12.19 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01750.01950.0185
10.970.02640.02380.02510.0066
32.920.03200.03110.03160.0131
98.770.06400.06290.06350.0450
296.300.22200.22400.22300.2045
888.890.93900.91380.92640.9079
2666.672.47022.44502.45762.4391
8000.003.97504.02203.99853.9800

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)78.1395.81027.182.4379.21127.4
Standard Deviation2.414.830.94.224.174.6
Coefficient of Variation(%)3.13.73.04.06.46.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-67.432039.61

Sensitivity

8.58 pg/ml (50 μl)；12.19 pg/ml (10 μl)

Standard curve range

10.97~8000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL20 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 121% to 128% with an overall mean recovery of 115%.

Specifications

Product Type

ELISA Kit

Product Name

Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit (EH0028)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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